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产品别名
Anti-PRDM14 Antibody
PRDM-14, PR domain zinc finger protein 14
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.41 |
| General description【一般描述】 | PRDM14 is an approximately 65 kDa transcriptional inhibitor that contains one histone methylating PR/SET domain (aa 266 - 367) and six zinc finger repeats (aa 400 - 568). PRDM14 is preferentially expressed in germ cells and undifferentiated embryonic stem cells in which it plays a central role in lineage specification, maintenance of pluripotency, and repression of somatic gene transcription. PRDM14 is upregulated in breast cancer and lymphoblastic leukemia. Recently, PRDM14 ensures naive pluripotency through dual regulation of signaling and epigenetic pathways in embryonic stem cells |
| Specificity【特异性】 | Human |
| Immunogen【免疫原】 | Human recombinant PRDM14. Epitope: N-terminus |
| Application【应用】 | Research Sub Category Pluripotent & Early Differentiation Research Category Stem Cell Research This Anti-PRDM14 Antibody is validated for use in Western Blotting and Chromatin Immunoprecipitation (ChIP) and ChIP-seq for the detection of PRDM14 . Chromatin Immunoprecipitation Analysis: A representative lot was used by an independent laboratory on H1 human ES cells (CHAN et al., 2013. STEM CELLS, 31:682–692) ChIPseq Analysis: A representative lot was used by an independent laboratory on H1 human ES cells (Chia et al., 2010. Nature. 316, VOL 468) |
| Quality【质量】 | Evaluated by Western Blotting in Human ES cell cell lysate. Western Blot Analysis: 1 µg/mL of this antibody detected PRDM14 in undifferentiated human ES cell lysate. |
| Physical form【外形】 | Format: Unpurified Raw rabbit polyclonal serum Unpurified |
| Other Notes【其他说明】 | Concentration: Please refer to lot specific datasheet. |
产品性质
| biological source【生物来源】 | rabbit |
| Quality Level【质量水平】 | 100 |
| antibody form【抗体形式】 | unpurified |
| antibody product type | primary antibodies |
| clone【克隆】 | polyclonal |
| species reactivity | human |
| technique(s) | ChIP: suitable (ChIP-seq) western blot: suitable |
| isotype【同位素/亚型】 | IgG |
| UniProt accession no.【UniProt登记号】 | Q9GZV8 |
| shipped in【运输】 | wet ice |
产品说明
| Target description【目标描述】 | ~64 kDa observed. Uncharacterized bands may be observed in some lysate(s). |
| Storage and Stability【储存及稳定性】 | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
| Storage Class Code【储存分类代码】 | 10 - Combustible liquids |
| WGK | WGK 1 |
