COL, S human cell line

human blood

Australian Aborigine/Caucasian

female

Suspension

Not specified

Lymphoblastoid

cell culture | mammalian: suitable

dry ice

Homozygous: No
Consanguineous: No

This cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.

A: 03 24
B: 07 40
HLA DRB1: 1501 1405
HLA DRB3: 202
HLA DRB5: 101
HLA DQAP: 1.2 1.3
HLA DQA1: 0102 0104
HLA DQB1: 0503 06
HLA DPB1: 0401 0501

RPMI 1640 (R0883) + 2mM Glutamine (G7513) + 20% Foetal Bovine Serum (FBS) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.

Maintain cultures between 3x10⁵ - 2x10⁶ cells/ml, 5% CO2, 37 ℃. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.

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HLA A: 3 24
HLA A ief:
HLA C: 7
HLA B: 7 40

This cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.