PNT2 Cell Line human

human prostate

Adherent

Not specified

Epithelial

Not specified

Not specified

cell culture | mammalian: suitable

dry ice

−196℃

Human prostate normal, immortalised with SV40

Established by immortalisation of normal adult prostatic epithelial cells by transfection with a plasmid containing SV40 genome with a defective replication origin. The primary culture was obtained from a prostate of a 33 year old male at post mortem. PNT2 cells contain the SV40 genome and express large T protein. They possess a well differentiated morphology with the expression of cytokeratin 8,18 and 19 with the latter being a feature of differentiated luminal cells of the glandular prostate. Cytokeratin 14, a marker of epithelial basal cells, is not expressed. The cells are non-tumourigenic in nude mice.

Biology of prostate cells, model of differentiated prostate epithelial cells.
PNT2 cell line has been used:

• to assess the effects of Verapamil, an L-type calcium channel blocker on autophagy
• to assess the cytotoxicity of aqueous dilutions of a universal self-priming dental adhesive (DA)
• to study the expression of soluble adenylyl cyclase (sAC) in the cells

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11,13
D13S317: 8,13
D16S539: 9
D5S818: 12,13
D7S820: 11
THO1: 6,9.3
TPOX: 8,9
vWA: 17

RPMI 1640 + 2mM Glutamine + 6 -10% Foetal Bovine Serum (FBS).

Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37℃.

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.