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Iscove′s Modified Dulbecco′s Medium
产品编号: | 4076803 |
规格: | With L-alanyl-L-glutamine and sodium bicarbonate, sterile-filtered, liquid, suitable for cell culture |
包装规格: | 500 ML |
产品类别: | 进口试剂 |
品牌: | SAFC |
优惠价: | 立即咨询 |
产品性质
Quality Level【质量水平】 | 400 |
sterility【无菌性】 | sterile-filtered sterile |
product line【产品线】 | AQmedia™ |
form【形式】 | liquid |
technique(s) | cell culture | mammalian: suitable |
impurities【杂质】 | Endotoxin, tested |
shipped in【运输】 | ambient |
storage temp.【储存温度】 | 2-8℃ |
基本信息
Components【组分】 | glucose: yes NaHCO3: yes stable glutamine: yes phenol red: yes |
General description【一般描述】 | This IMDM differs from the original formula, because it is supplemented with L-alanyl-L-glutamine dipeptide. Glutamine is notoriously unstable in cell culture. This dipeptide provides a more stable form of glutamine for use in cell cultures. |
Application【应用】 | Guilbert and Iscove demonstrated that precursor cells of erythrocytes and macrophages could be cultured in a totally defined serum free medium supplemented with albumin, transferrin, lecithin, and selenium. This medium is a modification of Dulbecco¢s Modified Eagle¢s Medium (DME) containing selenium, additional amino acids and vitamins, sodium pyruvate, HEPES buffer, and potassium nitrate instead of ferric nitrate. Further studies demonstrated that Iscove¢s Medium would support murine B lymphocytes, hemopoietic tissue from bone marrow, B cells stimulated with lipopolysaccharide, T lymphocytes, and a variety of hybrid cells. |
Linkage【联系】 | Need FBS? We can assist with your serum planning. Please visit the Serum Planner for more information. |
Legal Information【法律信息】 | AQmedia is a trademark of sigma-aldrich Co. LLC |
安全信息
Storage Class Code【储存分类代码】 | 10 - Combustible liquids |
WGK | WGK 1 |