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ExtrAvidin®-Agarose, high binding
| 产品编号： | 4076370 |
| 规格： | For purification of biotinylated macromolecules and complexes |
| 包装规格： | 1 ML，5 ML |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
产品性质
| form【形式】 | aqueous suspension suspension |
| feature【特点】 | For purification of biotinylated macromolecules and complexes |
| concentration【浓度】 | 50% |
| matrix【基质】 | 4% agarose (beaded) |
| matrix activation【基质活化】 | cyanogen bromide |
| storage temp.【储存温度】 | 2-8℃ |
基本信息
| General description【一般描述】 | ExtrAvidin® is a unique de-glycosylated Avidin modified form of an affinity purified egg white avidin. The ExtrAvidin is conjugated to cyanogen bromide-activated agarose beads. ExtrAvidin® is a tetrameric protein containing four biotin binding sites. The Avidin-Biotin high affinity interaction (Kd=10-15M) is considered one of the strongest non-covalent interactions known in nature. The use of Avidin-Biotin complex for affinity purification was described in the first half of 1970 and since then it was successfully utilized in numerous studies and biotechnological applications.1-3 This was achieved due to the ability to chemically coupe the small Biotin molecule with different binders, without disturbing its function or structure, thus allowing unique interaction with a variety of Avidin carriers including: protein or DNA molecules, Avidin protein bound to a solid surface matrix, reporter molecules, probes or carriers,. Among the known Avidin-Biotin interaction based applications are purification, enrichment, detection, amplification and other research medical and industrial processes. In addition, the ExtrAvidin® high specificity binding to Biotin, together with the low background staining, grant it significant advantage compared to the non-modified Avidin or Streptavidin produced by Streptomyces avidinii. |
| Application【应用】 | ExtrAvidin®-Agarose provides high affinity with high specificity binding to Biotin (also known as vitamin B7) including biotinylated proteins and biotin-tagged fusion proteins. The product may be used in various immunological techniques, including Immunoprecipitation and Immunoaffinity purification. |
| Caution【注意】 | DO NOT FREEZE |
| Physical form【外形】 | ExtrAvidin®-Agarose is provided as suspension at a 1:1 ratio, in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide as a preservative. |
| Legal Information【法律信息】 | ExtrAvidin is a registered trademark of sigma-aldrich Co. LLC |
产品说明
| Disclaimer【免责声明】 | This product is for research use only, not for drug, household, or other uses. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices. |
安全信息
| Storage Class Code【储存分类代码】 | 10 - Combustible liquids |
| WGK | WGK 3 |
