Aprotinin, Bovine, Recombinant, Nicotiana sp., Animal-Free-CAS 9087-70-1-Calbiochem

C₂₈₄H₄₃₂N₈₄O₇₉S₇

6511.44

MFCD00130541

2329949

Recombinant, bovine aprotinin supplied without animal-derived components. Aprotinin is a competitive, reversible inhibitor of proteolytic and esterolytic activity. A relatively heat- and acid-stable serine protease inhibitor. Forms a tight complex, blocking the active site of the enzyme. Effective at concentrations equimolar with protease. Inhibits several proteases, including coagulation factors in the prephase of blood clotting, tissue and leukocytic proteinases, chymotrypsin, trypsin (K_d = 5 x 10^-14 M), plasmin (K_d = 2.3 x 10^-10 M), and kallikrein (K_d = 1 x 10^-7 M). Proteases not inhibited by aprotinin include Factor Xa, thrombin, pepsin, papain, and carboxypeptidases A and B. Useful for protein purification and for extending the life of cells in culture by preventing proteolytic damage.

Product does not compete with ATP.
Reversible: yes
Kd = 5 x 10-14 M, 2.3 x 10-10 M, 1 x 10-7 M against trypsin, plasmin, and kallikrein, respectively
Primary Target
chymotrypsin
Cell permeable: no

Toxicity: Standard Handling (A)

One TIU (Trypsin Inhibitory Unit) is defined as the amount of aprotinin or product or inhibitor that will decrease the activity of 2 trypsin units by 50% where one trypsin unit will hydrolyze 1 µmol BAPNA per min at 25°C, pH 7.8.

Contains no animal-derived components.

Deutscher, M.P., 1990. Methods Enzymol.182, 83.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

200

expressed in Nicotiana

≥95% (SDS-PAGE)

powder

>5 TIU/mg protein

Calbiochem^®

OK to freeze

white to yellow

wet ice

2-8℃

25 mg in Glass bottle
1, 5 mg in Plastic ampoule
Please refer to vial label for lot-specific concentration.

12 - Non Combustible Liquids

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