16:0-16 Doxyl PC

C₄₆H₉₀N₂O₁₀P

862.19

NA.25

1-palmitoyl-2-stearoyl-(16-doxyl)-sn-glycero-3-phosphocholine (16:0-16 Doxyl PC) is a spin labelled phospholipid.
Avanti′s nitroxide spin product listing is a group of compounds designed to act as membrane probes. A variety of positions down the hydrophobic chain are labeled with the nitroxide functional groups to allow probing the membrane at various depths. These compounds have been synthesized from 1-palmitoyl-2-hydroxy-sn-glycerol-3-phosphocholine with the product being purified by column chromatography. Various n-doxyl phosphocholines have been recently used as biophysical tools to elucidate membrane trafficking with phosphatidylinositol transfer proteins and as fluorescent quenchers in lipid bilayer structural studies.

1-palmitoyl-2-stearoyl-(16-doxyl)-sn-glycero-3-phosphocholine (16:0-16 Doxyl PC) may be used:

• in bilayers or membranes to study the distribution of the lipid-attached doxyl electron paramagnetic resonance (EPR) spin label
• as spin-labelled lipid in multilamellar vesicles (MLVs) to measure membrane fluidity
• as a spin-labeled deep quencher in small unilamellar vesicles (SUVs) for nitroxide lipid quenching experiments

Product use: To prevent aggregation, prepare water-based solutions of 2 mM stock solutions of n-DOXYL PCs and store in plastic. Dilute stock solutions to 0.03- 0.1 mM solutions for EPR studies. For liposome preparations in fluorescent quenching measurements, dissolve the doxyl lipid in 150 μl absolute ethanol for a concentration of 40.3 mM , Additional supplemental information.

>99% (TLC)

Avanti Polar Lipids 810604C

1 mg/mL (810604C-1mg)

dry ice

−20℃

5 mL Clear Glass Sealed Ampule (810604C-1mg)

GHS06,GHS08

Danger

H302 - H315 - H319 - H331 - H336 - H351 - H361d - H372 - H412

P201 - P273 - P301 + P312 + P330 - P302 + P352 - P304 + P340 + P311 - P308 + P313

Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Carc. 2 - Eye Irrit. 2 - Repr. 2 - Skin Irrit. 2 - STOT RE 1 - STOT SE 3

Central nervous system

6.1D - Non-combustible, acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

WGK 3