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β-羟丁酸脱氢酶 来源于球形红假单胞菌

β-Hydroxybutyrate Dehydrogenase from Rhodopseudomonas sphaeroides
产品编号:3411518
规格:Type V, lyophilized powder, 250-750 units/mg protein
CAS NO:9028-38-0
包装规格:25 UNITS
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品编号包装单位单价(元)国内现货国外库存询价单
341151825 UNITS15500
产品别名

9028-38-0

β-羟丁酸脱氢酶 来源于球形红假单胞菌

β-Hydroxybutyrate Dehydrogenase from Rhodopseudomonas sphaeroides

(R)-3-Hydroxybutanoate:NAD+ oxidoreductase

3-HBDH

(R)-3-羟基丁酸:NAD+ 氧化还原酶

基本信息
Enzyme Commission (EC) Number【酶学委员会(EC)编号】
1.1.1.30 ( BRENDA | IUBMB )
EC number【EC 号】
232-830-6
MDL number
MFCD00130633
NACRES
NA.54
Application【应用】
适用于通过 Williamson, D. H., and Mellanby, J., Methods of Enzymatic Analysis, Bergmeyer, H., ed., 2nd edition, 4, 1836 (1974) 中介绍方法的测定乙酰乙酸酯和 D(-)-3-羟基丁酸酯。
Biochem/physiol Actions【生化/生理作用】
In mammalian systems, β-hydroxybutyrate dehydrogenase is localized on the inner mitochondrial membrane and requires phosphatidyl choline for activity. In contrast, the enzyme from Rhodopseudomonas is a soluble cytosolic enzyme that does not require a phospholipid allosteric activator. The enzyme is required for the utilization of ketone bodies as a source of metabolic energy. It catalyzes the oxidation of 3-hydroxybutyrate to acetoacetate, the first step in the conversion of ketone bodies to citric acid, which is then further metabolized via the tricarboxylic acid cycle (Krebs cycle).
Unit Definition【单位定义】
One unit will oxidize 1.0 μmole of D-β-hydroxybutyrate to acetoacetate per min at pH 7.8 at 37 ℃.
Physical form【外形】
含 Tris 缓冲盐的冻干粉
产品性质
Quality Level【质量水平】
200
type【类型】
Type V
form【形式】
lyophilized powder
Specific Activity
250-750 units/mg protein
purified by【纯化方式】
chromatography
foreign activity【异质活性】
lactate dehydrogenase ≤0.05%
malate dehydrogenase ≤0.1%
storage temp.【储存温度】
−20℃
packaging【包装】
25 units in serum bottle
安全信息
Storage Class Code【储存分类代码】
12 - Non Combustible Liquids
WGK
WGK 3
Personal Protective Equipment【个人防护装备】
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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