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亲和素 来源于鸡蛋白
Avidin from egg white
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产品编号 | 包装单位 | 单价(元) | 国内现货 | 国外库存 | 询价单 |
3411039 | 5 MG | 2910 |
产品别名
1405-69-2
亲和素 来源于鸡蛋白
Avidin from egg white
基本信息
EC number【EC 号】 | 215-783-6 |
MDL number | MFCD00130572 |
NACRES | NA.56 |
General description【一般描述】 | Avidin is a homotetrameric glycoprotein found in the egg white of birds, reptiles and amphibians. Each subunit is 16 kDa, singly glycosylated and binds to a molecule of biotin with greater affinity and specificity. Recombinant avidin from corn is similar to avidin from egg white in terms of properties like isoelectric point (pI) and antigenic properties except for the molecular weight. Avidin from corn has low molecular weight than chicken egg-derived avidin. This might be due to less complex glycosylation composition in plants. Commercial production of avidin from corn has certain advantages in terms of availability of greater biomass and avoiding the co-purification of animal virus. |
Application【应用】 | Avidin forms an extremely strong complex with d-biotin (Kd ~ 10-15). This activity has made both avidin and biotin extremely useful labels in immunochemical methods of detection and quantitation. 亲和素蛋白用于一项分析中,该分析使用官能化氙作用检测生物素-亲和素结合的生物传感器。使用80nmol的鸡蛋白。 |
Unit Definition【单位定义】 | One unit will bind 1.0 μg of d-biotin. |
Preparation Note【制备说明】 | 亲和纯化 |
产品性质
Quality Level【质量水平】 | 200 |
recombinant【重组】 | expressed in corn |
Assay【测定】 | ≥80% protein basis (biuret) |
form【形式】 | powder |
Specific Activity | ≥12 units/mg protein (E |
mol wt【分子量】 | glycoprotein 66 kDa subunit 16 kDa |
technique(s) | ELISA: suitable immunoblotting: suitable immunoelectrophoresis: suitable |
UniProt accession no.【UniProt登记号】 | P02701 |
storage temp.【储存温度】 | 2-8℃ |
Gene Information | chicken ... AVD(396260) |
packaging【包装】 | 5 mg in glass bottle |
安全信息
Storage Class Code【储存分类代码】 | 13 - Non Combustible Solids |
WGK | WGK 3 |
Personal Protective Equipment【个人防护装备】 | Eyeshields, Gloves, type N95 (US) |