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产品性质
biological source【生物来源】 | rat pancreas |
growth mode【生长模式】 | Semi-adherent aggregates |
karyotype【核型】 | Not specified |
morphology【形态学】 | Pancreas cells |
products【产品】 | Amylase and exocrine enzymes |
receptors【受体】 | Gastrin/CCKB, Epidermal growth factor (EGF), Somatostatin |
technique(s) | cell culture | mammalian: suitable |
relevant disease(s)【相关疾病】 | cancer |
shipped in【运输】 | dry ice |
storage temp.【储存温度】 | −196℃ |
基本信息
Cell Line Origin【细胞系来源】 | Rat exocrine pancreatic tumour |
Cell Line Description【细胞系描述】 | Derived from a transplantable tumour of a rat exocrine pancreas. The line is tumourigenic in nude mice, and shows significant secretion of amylase and other exocrine enzymes. |
Application【应用】 | AR42J has been used to obtain a tumor mouse model by inoculating it subcutaneously in a female, athymic nude mouse. It has also been used to perform imaging studies. |
Culture Medium【培养基】 | RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS). |
Subculture Routine【传代培养常规】 | Cells grow in hollow spheroid colonies that can attach loosely. Split cultures 1:2 every 48 hours into fresh flasks, maintain cultures between 1-9x100,000 cells/ml. Adherent cells should be dislodged using 0.2% EDTA. N.B. High cell viability cannot be ex |
Other Notes【其他说明】 | Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information. |