Anti-STIM1 (N-terminal) 兔抗

NA.41

STIM1 (stromal interaction molecule 1) is a conserved single-pass transmembrane protein, which is expressed ubiquitously in a wide variety of human primary and transformed cell types. STIM1 is modified by phosphorylation and N-linked glycosylation. STIM1 localized predominantly in the membrane of the endoplasmic reticulum (ER). It contains an N-terminal EF hand motif located in the ER lumen and appears to function as a sensor of ER Ca²⁺ levels.

synthetic peptide corresponding to amino acids 61-74 of human STIM1, conjugated to KLH via a C-terminal cysteine residue. The corresponding sequence is identical in rat and mouse.

Anti-STIM1 (N-terminal) antibody produced in rabbit has been used in:

• western blotting
• immunoprecipitation
• immunofluorescence

STIM1 (stromal interaction molecule 1) is required for the activation of store-operated Ca²⁺ influx. Overexpression of STIM1 and Orai1 markedly increases the calcium release-activated calcium (CRAC) current (I-CRAC).

Solution in 0.01 M phosphate buffered saline, 7.4 pH, containing 15 mM sodium azide.

rabbit

200

unconjugated

affinity isolated antibody

primary antibodies

polyclonal

buffered aqueous solution

antigen 90 kDa

human, rat, mouse

~1 mg/mL

immunoprecipitation (IP): 2.5-5 μg using extracts of rat PC12 cells.
indirect immunofluorescence: 5-10 μg/mL using human HeLa cells.
western blot: 2-4 μg/mL using whole extract of mouse 3T3 cells.

Q13586

dry ice

−20℃

human ... STIM1(6786)
mouse ... Stim1(20866)
rat ... Stim1(117086)

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