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Anti-phospho-PKB (pThr308) antibody produced in rabbit
| 产品编号： | 3385504 |
| 规格： | IgG fraction of antiserum, buffered aqueous solution |
| 包装规格： | 0.2 ML |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
产品别名
Anti-phospho-PKB (pThr308) antibody produced in rabbit
Anti-phospho-Akt
基本信息
| MDL number | MFCD02686506 |
| NACRES | NA.44 |
| General description【一般描述】 | Protein kinase B (PKB, also known as Akt) is a serine/threonine protein kinase and a key regulator of signal transduction processes. Important processes such as cell cycle, proliferation and apoptosis are regulated by PKB in response to stimuli such as growth factors, insulin and cytokines. Three isoforms of PKB have been identified, α, β and γ. PKB α is reportedly overexpressed in breast cancer cell lines, whereas PKB β is overexpressed in ovarian and pancreatic cancers. On cell stimulation by growth factors or insulin, PKB α translocates to the cell membrane, where it undergoes full activation by phosphorylation at Thr308 and Ser 473, by PDK1 and PDK2. PKB α plays a crucial role in several downstream pathways involved in glycogen synthesis, protein translation and cell proliferation. PKB is also a potent inhibitor of apoptotic cell death by suppressing BAD-induced death Anti-phospho PKB (pThr308) recognises PKB phosphorylated at threonine 308 (56 kDa). |
| Immunogen【免疫原】 | synthetic phosphopeptide corresponding to the pThr308 region of human PKBα (amino acids 301-315). |
| Application【应用】 | Anti-phospho PKB (pThr308) may be used for detection and localization of phospho-PKB by immunoblotting. A minimum dilution of 1:1000 may be used for detection by immunoblotting in PDGF-treated mouse NIH3T3 cells and peroxide-treated Rat-1 fibroblast cell line. |
| Physical form【外形】 | 0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。 |
产品性质
| biological source【生物来源】 | rabbit |
| Quality Level【质量水平】 | 200 |
| conjugate【偶联物】 | unconjugated |
| antibody form【抗体形式】 | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| clone【克隆】 | polyclonal |
| form【形式】 | buffered aqueous solution |
| mol wt【分子量】 | antigen 56 kDa |
| species reactivity | rat, mouse |
| technique(s) | microarray: suitable western blot: 1:1,000 using a whole extract of PDGF-treated mouse fibroblast NIH3T3 cells and a whole extract of H2O2-treated rat fibroblast Rat-1 cells |
| UniProt accession no.【UniProt登记号】 | P31750 |
| shipped in【运输】 | dry ice |
| storage temp.【储存温度】 | −20℃ |
| Gene Information | human ... AKT1(207) mouse ... Akt1(11651) rat ... Akt1(24185) |
产品说明
| Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
| Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
| WGK | nwg |
