Anti-Mouse IgG-Atto 655 antibody produced in goat

NA.46

Anti-mouse IgG (whole molecule) (Sigma, M8645) is developed in goat using purified mouse IgG as the immunogen. Affinity isolated antigen specific antibody is purified from goat anti-mouse IgG antiserum to remove essentially all goat serum proteins, including immunoglobulins, which do not specifically bind to mouse IgG. The antibody preparation is solid phase adsorbed with human serum proteins to ensure minimal cross reactivity. Goat anti-mouse is conjugated to Atto 655 NHS (Abs.max. 663 nm; Em.max. 684 nm),(Cat. No. 76245), then further purified via gel permeation chromatography and dialysis to remove unbound Atto-Dye.

purified mouse IgG

Atto 655-goat anti-Mouse IgG - Atto 655 antibody has been used for immunofluorescence applications.

Unconjugated dye ≤5% of total fluorescence.

100

goat

expressed in HEK 293 cells

Atto 655 conjugate

affinity isolated antibody

secondary antibodies

polyclonal

50% glycerol

mouse

~1 mg/mL IgG

immunofluorescence: suitable

λ_ex 655 nm; λ_em 677 nm in PBS

wet ice

−20℃

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

GHS07

Warning

H319

P305 + P351 + P338

Eye Irrit. 2

12 - Non Combustible Liquids

WGK 3

Eyeshields, Gloves