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抗 谷胱甘肽-S-转移酶
Anti-Glutathione-S-Transferase (GST)–Agarose antibody produced in rabbit
产品编号: | 3365109 |
规格: | IgG fraction of antiserum, PBS suspension |
包装规格: | 1 ML |
产品类别: | 进口试剂 |
品牌: | Sigma-Aldrich |
优惠价: | 立即咨询 |
产品别名
抗 谷胱甘肽-S-转移酶
Anti-Glutathione-S-Transferase (GST)–Agarose antibody produced in rabbit
Anti-Glutathione-S-Transferase
Anti-GST
抗 GST
基本信息
NACRES | NA.46 |
General description【一般描述】 | 该抗体对日本血吸虫 谷胱甘肽-S-转移酶的天然型和变性-还原型均具有特异性 。抗 GST 可用于各种免疫分析,以鉴定 GST 融合蛋白的表达。 Glutathione-S-transferases (GSTs) are dimeric proteins, which belongs to phase II detoxification enzymes family. The highly polymorphic human cytosolic GSTs are divided into six classes, such as, α, μ, ω, π, θ, and ζ. |
Immunogen【免疫原】 | 日本血吸虫 的重组 GST 在 大肠杆菌中表达 。 |
Application【应用】 | Immunoprecipitation was performed on NIH 3T3 cells transiently transfected with GST fusion proteins. IP was performed using agarose-linked rabbit anti-GST IgG with mixing for 2 hours at 4 degrees. Rabbit Anti-Glutathione-S-Transferase (GST)-Agarose antibody can be used for ELISA, immunoaffinity purification and immunoprecipitation assays. |
Biochem/physiol Actions【生化/生理作用】 | Glutathione-S-transferases (GSTs) can catalyse the conjugation of glutathione (GSH) to various endogenous and exogenous electrophilic compounds. The π and μ classes of GSTs controls the mitogen-activated protein (MAP) kinase pathway. It can also serve as transport proteins. |
Physical form【外形】 | Suspension in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide |
Preparation Note【制备说明】 | Prepared by coupling cyanogen bromide-activated agarose at 7-8 mg antibody per mL resin volume. |
产品性质
biological source【生物来源】 | rabbit |
Quality Level【质量水平】 | 200 |
conjugate【偶联物】 | agarose conjugate |
antibody form【抗体形式】 | IgG fraction of antiserum |
antibody product type | primary antibodies |
clone【克隆】 | polyclonal |
form【形式】 | PBS suspension |
mol wt【分子量】 | antigen 27.5 kDa |
should not react with | rabbit, human, rat, bovine, porcine |
technique(s) | ELISA: suitable immunoprecipitation (IP): suitable |
shipped in【运输】 | wet ice |
storage temp.【储存温度】 | 2-8℃ |
产品说明
Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
WGK | WGK 1 |