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Anti-CSTF1 (C-terminal) antibody produced in rabbit
| 产品编号： | 3355605 |
| 规格： | ~1 mg/mL, affinity isolated antibody, buffered aqueous solution |
| 包装规格： | 200 μL |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
产品别名
Anti-CSTF1 (C-terminal) antibody produced in rabbit
Anti-Cleavage stimulation factor, 3-prime PRE-RNA, subunit 1, 50-KD
Anti-CstF-50
Anti-CstFp50
基本信息
| NACRES | NA.41 |
| General description【一般描述】 | Cleavage stimulation factor 1 (CSTF1) contains seven WD-40 repeats. The CSTF1 gene is mapped to human chromosome 20q13.2-q13.31. |
| Specificity【特异性】 | Anti-CSTF1 (C-terminal) specifically recognizes human CSTF1 (also known as Cleavage stimulation factor). |
| Application【应用】 | Anti-CSTF1 (C-terminal) antibody produced in rabbit is suitable for immunoprecipitation at a working amount of 5-10μg using K562 lysates and western blotting at a concentration of 2-4μg/mL using K562 lysates. |
| Biochem/physiol Actions【生化/生理作用】 | Cleavage stimulation factor (50kDa) subunit is a protein encoded by the CSTF1 gene in humans. It is one of the multiple factors that plays a significant role during polyadenylation of mammalian pre-mRNAs. The mRNA polyadenylation factor CstF interacts with the BRCA1-associated protein BARD1 resulting in repression of the nuclear mRNA polyadenylation machinery. It participates in the regulation of endogenous transcripts during DNA-damage. CSTF1 is required for in vitro cleavage activity. The WD-40 repeats are crucial for the interaction with CSTF3. CSTF1 also interacts directly with the RNA polymerase II CTD, suggesting a role in linking transcription and mRNA 3′-ends processing. |
| Physical form【外形】 | 0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。 |
产品性质
| biological source【生物来源】 | rabbit |
| Quality Level【质量水平】 | 200 |
| conjugate【偶联物】 | unconjugated |
| antibody form【抗体形式】 | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone【克隆】 | polyclonal |
| form【形式】 | buffered aqueous solution |
| mol wt【分子量】 | antigen ~48 kDa |
| species reactivity | human |
| concentration【浓度】 | ~1 mg/mL |
| technique(s) | immunoprecipitation (IP): 5-10 μg using K562 lysates western blot: 2-4 μg/mL using K562 lysates |
| UniProt accession no.【UniProt登记号】 | Q05048 |
| shipped in【运输】 | dry ice |
| storage temp.【储存温度】 | −20℃ |
| Gene Information | human ... CSTF1(1477) mouse ... Cstf1(67337) rat ... Cstf1(311670) |
产品说明
| Storage and Stability【储存及稳定性】 | Store at –20 ℃. For continuous use, the product may be stored at 2–8 ℃ for up to one month. For extended storage, freeze in working aliquots at –20 ℃. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours. |
| Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
