Anti-APC (C-terminal) antibody produced in rabbit

NA.41

Adenomatous Polyposis Coli (APC) is a tumor suppressor gene. The APC gene encodes a large multifunctional protein that has critical functions in cell migration, adhesion and proliferation. It is a shuttling nucleo-cytoplasmic protein expressed in various tissues and its expression in the colorectal epithelium contributes to its normal growth and differentiation.

synthetic peptide corresponding to a sequence at the C-terminal region of human APC, conjugated to KLH. The corresponding sequence is identical in human APC isoform b and highly conserved (single amino acid substitution) in rat and mouse APC.

Anti-APC (C-terminal) antibody produced in rabbit has been used in:

• immunoblotting
• immunofluorescence
• immunohistochemistry

Adenomatous Polyposis Coli (APC) has a key role in the proteasome-mediated degradation of β-catenin. APC mutations cause accumulation of β-catenin in the nucleus leading to activation of LEF-1 and/or TCF, and the induction of target genes such as the oncogene c-myc. It is involved in regulation of mitotic chromosome separation and stability.

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

rabbit

unconjugated

affinity isolated antibody

primary antibodies

polyclonal

buffered aqueous solution

antigen ~300 kDa

human

~1.0 mg/mL

immunoblotting: 3-6 μg/mL using lysates of HeLa or SW620 cells.
immunohistochemistry: 10-20 μg/mL using formalin-fixed, paraffin-embedded human colon or duodenum.
indirect immunofluorescence: 2-4 μg/mL using Caco-2 cells.

P25054

dry ice

−20℃

human ... APC(324)

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