Anti-ALIX (C-terminal) antibody produced in rabbit

NA.41

The gene PDCD6IP (programmed cell death 6 interacting protein), also known as ALIX (apoptosis-linked gene 2-interacting protein X), is mapped to human chromosome 3p22.1 The protein has a crescent-shaped Bro1 domain, V-shaped V domain and a proline-rich region.

Anti-ALIX (C-terminal) recognizes human and mouse ALIX.

peptide corresponding to a sequence at the C-terminal region of human ALIX/ PDCD6IP, conjugated to KLH. The corresponding sequence is identical in mouse, pig, monkey, bovine and dog and differs by a single amino acid in rat.

Anti-ALIX (C-terminal) antibody produced in rabbit may be used in:

• immunoblotting
• immunoprecipitation
• immunofluorescence

Apoptosis-linked gene 2-interacting protein X (ALIX) protein binds to endophilin proteins that regulate membrane shape during endocytosis. Over-expression of the ALIX gene and endophilin leads to cytoplasmic vacuolization, which may be a reason for protection against cell death.
PDCD6IP (programmed cell death 6 interacting protein) interacts with PDCD6, a protein involved in apoptosis. It also participates in intraluminal endosomal vesicle biogenesis, viral budding and cytokinetic abscission. PDCD6IP is a part of the ESCRT (endosomal sorting complexes required for transport) machinery.

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.

rabbit

200

unconjugated

affinity isolated antibody

primary antibodies

polyclonal

buffered aqueous solution

antigen ~95 kDa

mouse, human

~1.0 mg/mL

immunoprecipitation (IP): 1-2 μg using lysates of human Jurkat cells
indirect immunofluorescence: 2.5-5 μg/mL using human HeLa cells
western blot: 1-2 μg/mL using whole extracts of mouse A20 cells

Q8WUM4

dry ice

−20℃

human ... PDCD6IP(10015)
mouse ... Pdcd6ip(18571)

For continuous use, store at 2-8℃ for up to one month. For extended storage freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

WGK 2