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REDExtract-N-AmpTM PCR ReadyMixTM

产品编号:3214682
规格:Ready-to-use 2X PCR Master Mix with Loading Dye
包装规格:1.2 ML,12 ML,125 ML
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
32146821.2 ML2450
321468212 ML21600
3214682125 ML138980
基本信息
NACRES
NA.55
General description【一般描述】
REDExtract-N-Amp PCR反应混合物是包含缓冲液、盐、dNTP和REDTaq DNA聚合酶的PCR预混液。
该PCR ReadyMix要与Sigma的Extract-N-Amp植物PCR试剂盒以及Extract-N-Amp组织PCR试剂盒一起使用。所有Extract-N-Amp试剂盒均可为每次提取进行一次PCR反应。然而,如果需要更多的PCR反应,则可能需要补充额外的PCR ReadyMix。REDExtract-N-Amp PCR ReadyMix中含有一种惰性染料,可作为示踪染料以实现方便地将PCR产物加载到琼脂糖凝胶上进行分析。
Application【应用】
REDExtract-N-Amp PCR Master Mix has been used:
  • for the genotyping of fixed zebrafish embryo samples
  • in polymerase chain reaction (PCR) amplification of the DNA isolated from lichen family using the REDExtract-N-Amp Plant PCR Kit
  • in the amplification of DNA isolated from single yeast colonies and fungal spores using REDExtract-N-Amp seed PCR kit.

REDExtract-N-Amp PCR Master Mix is also suitable for colony PCR.
Features and Benefits【特点和优势】
  • A Gel-ready- No loading buffer or tracking dye needs to be added. The PCR product is loaded directly into an agarose gel after amplification. The red tracer migrates at approximately the same rate as a 125bp fragment (slightly faster than bromophenol blue).
  • Convenience- Hot-start feature in JumpStart products allows reaction set up at room temperature, which can be beneficial when working with multiple samples. Ready-mix format reduces setup time.
  • Reproducibility- Reduces the risk of contamination from multiple pipet steps and provides consistent performance across both reactions and time.
  • High Specificity & Yield- JumpStart Taq DNA polymerase, an antibody inactivated hot-start enzyme, is designed to minimize non-specific amplification while increasing target yield & specificity.
Legal Information【法律信息】
本产品的使用受到如下一项或多项美国专利及其对应的境外专利声明保护:5,789,224, 5,618,711, 6,127,155以及与届满的美国专利号5,079,352对应的境外专利声明。购买本产品,即相当于依照境外的专利声明获得了一份受限制、不可转让的使用许可,即将此等数量的产品用于购买者内部的研究用途。我们未明确表示、暗示或以禁止反言的形式授予您任何其他专利声明下的权利、进行任何专利方法申请的权利、进行任何形式的商业服务的权利,包括但不限于出于收费或其他商业考虑而报告购买者的研究活动结果的权利。本产品仅适合于研究用途。如需用于Roche专利许可的诊断用途,需另外征得Roche许可。有关购买许可的更多信息,可联系Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA获取。
Extract-N-Amp is a trademark of sigma-aldrich Co. LLC
JumpStart is a trademark of sigma-aldrich Co. LLC
REDExtract-N-Amp is a trademark of sigma-aldrich Co. LLC
ReadyMix is a trademark of sigma-aldrich Co. LLC
产品性质
Quality Level【质量水平】
200
usage【用途】
sufficient for 100 reactions
1.2 mL sufficient for 100 amplifications
sufficient for 1000 reactions
12 mL sufficient for 1000 amplifications
sufficient for 10000 reactions
125 mL sufficient for 10000 amplifications
feature【特点】
dNTPs included
hotstart
color【颜色】
red
application(s)
agriculture
shipped in【运输】
wet ice
storage temp.【储存温度】
−20℃
安全信息
Storage Class Code【储存分类代码】
10 - Combustible liquids
WGK
WGK 3
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