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pBR322 Hae III Digest
基本信息
NACRES | NA.25 |
General description【一般描述】 | Sigma′s pBR322 Hae III Digest contains 22 fragments from 8 - 587 bp. Ten ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel. |
Application【应用】 | Suitable for size determination of dsDNA by electrophoresis with either agarose or polyacrylamide gels. |
Components【组分】 | 10mM Tris-HCl 中的溶液,pH 8.0,1mM EDTA |
Other Notes【其他说明】 | Contains 22 fragments, 8–587 bp For optimal resolution, the recommended agarose gel concentration is 2.5. |
Preparation Note【制备说明】 | Plasmid pBR322 DNA was digested with Hae III to completion, then purified by phenol extraction. |
产品性质
Quality Level【质量水平】 | 100 |
form【形式】 | liquid |
usage【用途】 | 1000 uses (0.5 mg size) 200 uses (0.1 mg size) |
suitability【适用性】 | suitable for electrophoresis (DNA) |
storage temp.【储存温度】 | −20℃ |
安全信息
Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |