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产品性质
biological source【生物来源】 | mouse nerve |
growth mode【生长模式】 | Adherent |
karyotype【核型】 | Unstable |
morphology【形态学】 | Neuroblast |
products【产品】 | Acetylcholinesterase, choline acetylase, tyrosine hydroxylase |
receptors【受体】 | Not specified |
technique(s) | cell culture | mammalian: suitable |
relevant disease(s)【相关疾病】 | cancer |
shipped in【运输】 | dry ice |
storage temp.【储存温度】 | −196℃ |
基本信息
Cell Line Origin【细胞系来源】 | Mouse C-1300 Neuroblastoma |
Cell Line Description【细胞系描述】 | Cloned from the C1300 mouse neuroblastoma line. Adapted to culture by alternate in vivo and in vitro passage. Cells have characteristics of mature neurons and secrete the enzymes for the synthesis of the neurotransmitters, choline acetylase and tyrosine hydroxylase. Cells are susceptible to VSV, HSV and Vaccinia viruses. |
Application【应用】 | Tumourigenicity, enzymatic and virus studies: VSV, HSV, vaccinia virus |
Culture Medium【培养基】 | Ham′s F10 + 2mM Glutamine + 15% Horse Serum (HS) + 2.5% Foetal Bovine Serum (FBS). |
Subculture Routine【传代培养常规】 | Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 3-5x10,000 cells/cm2 using 0.25% trypsin; 5% CO2; 37℃. |
Other Notes【其他说明】 | Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information. |