Monoclonal Anti-SSEA-5 antibody produced in mouse

NA.41

Undifferentiated human embryonic stem cells

Suggested starting dilutions are as follows: ICC/IF: 1:50-1:1000, Flow: 1:50. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Stage-specific embryonic antigen (SSEA)-5 is an H type-1 glycan that is highly and specifically expressed on human embryonic stem cells (hESCs), the human blastocyst inner cell mass and undifferentiated human pluripotent stem cells (hPSCs). Expression of SSEA-5 in hESCs and hPSCs rapidly decreases upon differentiation and is considered an indicator of undifferentiated PSCs. This SSEA-5 antibody is capable of identifying undifferentiated pluripotent stem cells in heterogeneous populations, and when used in conjunction with other pluripotency surface markers (CD9 and CD90 or CD50 and CD200), it can be used to immunodeplete residual teratoma-causing cells from incompletely differentiated embryonic stem cell cultures.

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Purification: Protein G affinity purified

Phosphate-buffered saline, no preservative added.

100

mouse

unconjugated

affinity isolated antibody

primary antibodies

8e11, monoclonal

buffered aqueous solution

human

1mg/mL

flow cytometry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable

IgG

wet ice

−20℃

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

WGK 1