Monoclonal Anti-Rat Kappa Light Chains (1a + 1b) antibody produced in mouse

MFCD00164734

NA.46

Monoclonal anti-Rat Light Chain (1a+1b) (mouse IgG1 isotype) is derived from the RT-39 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with purified rat IgG. An immunoglobulin (Ig) has two heavy chain and two light chain connected by disulfide bond. It is a glycoprotein. IgG is a major class of immunoglobulin. Rat has five immunoglobulin classes- IgM, IgG, IgA, IgD and IgGE. IgG has four subclasses- IgG1, IgG2a, IgG2b and IgG2c. Rat immunoglobulins have either κ or λ light chains. Greater than 90% of normal rat immunoglobulins and myelomas contain light chains.

The antibody reacts with an epitope on the rat κ light chain, which is shared by both the 1a and 1b allotypes in various rat immunoglobulin classes and subclasses. No cross-reactivity is observed with rat λ light chains or with IgG from human, mouse, bovine, rabbit, goat, sheep, horse, dog, chicken, pig or cat. The product shows weak cross-reaction with guinea pig IgG.

Purified rat IgG

Monoclonal Anti-Rat κ Light Chains (1a + 1b) antibody has been used in capture enzyme linked immunosorbent assay (ELISA), immunoblotting, dot blot and immunocytochemistry.
Monoclonal Anti-Rat Kappa Light Chains (1a + 1b) antibody produced in mouse has been used in:

• enzyme-linked immunosorbent assay (ELISA)
• immunoblotting
• dot blot
• immunocytochemistry

Immunoglobulin G (IgG) mainly helps in immune defense. It participates in hypersensitivity type II and type III reactions. It helps in opsonization, complement fixation and antibody dependent cell mediated cytotoxicity.

200

mouse

unconjugated

ascites fluid

secondary antibodies

RT-39, monoclonal

antigen 25 kDa

15 mM sodium azide

capture ELISA: suitable
dot blot: suitable
immunocytochemistry: suitable
indirect ELISA: 1:2,000
western blot: suitable using denatured and reduced normal serum or myeloma protein

IgG1

dry ice

−20℃

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

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