您的位置:首页 > 产品中心 > Anti-HMG-1 antibody, Mouse monoclonal antibody produced in mouse
Anti-HMG-1 antibody, Mouse monoclonal antibody produced in mouse
产品编号: | 3146466 |
规格: | clone HAP46.5, purified from hybridoma cell culture |
包装规格: | 100 μL,200 μL |
产品类别: | 进口试剂 |
品牌: | Sigma-Aldrich |
优惠价: | 立即咨询 |
产品别名
Anti-HMG-1 antibody, Mouse monoclonal antibody produced in mouse
Anti-High-Mobility-Group protein
Anti-Amphoterin
Anti-HMGB1
基本信息
MDL number | MFCD02263062 |
NACRES | NA.41 |
General description【一般描述】 | Monoclonal Anti-HMG-1 (mouse IgG1 isotype) is derived from the HAP46.5 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mouse immunized with purified human recombinant HMG-1. HMG-1 (High-Mobility-Group protein, amphoterin) is a highly conserved protein with more than 95% amino acid identity between rodent and human HMG-1. It is a prevalent non-histone chromatin component and a non-sequence specific DNA binding protein. HMG-1 consists of two homologous HMG boxes rich in basic amino acids and an acidic tail at the carboxy-terminus. High-mobility group-1 protein (HMG-1) or amphoterin is a nuclear DNA-binding protein that facilitates the bending and transcription of DNA. It also has a pivotal role in the regulation of chromatin structure, DNA replication, ligation and repair. Monoclonal anti-HMG-1 antibody can be used as a vital tool to study the biology of transcription. It can also be used in indirect ELISA and immunoblotting (25kD approx.). Mouse anti-HMG-1 antibody reacts specifically with HMG-1 of canine, mouse, chicken, rat and human. |
Immunogen【免疫原】 | purified recombinant human HMG-1. |
Application【应用】 | Monoclonal anti-HMG-1 antibody can be used in immunofluorescent staining. It can also be used in microarray and western blotting. Monoclonal Anti-HMG-1 antibody is suitable for use in immunochemical applications such as ELISA and immunoblotting (approx. 25 kDa). |
Biochem/physiol Actions【生化/生理作用】 | High-Mobility-Group protein, amphoterin, HMG-1 is involved in the regulation of chromatin structure as well as being involved, either as positive or negative factors with various aspects of DNA replication, transcription, repair, and ligation. HMG-1, identified as a membrane associated protein termed “amphoterin,” mediates neurite outgrowth, tumor outgrowth, and metastasis. It participates in plasminogen activation and is recognized as a late mediator of endotoxin lethality in mice. |
Physical form【外形】 | Solution in 0.01 M phosphate buffered saline containing 15 mM sodium azide. |
产品性质
Quality Level【质量水平】 | 200 |
biological source【生物来源】 | mouse |
conjugate【偶联物】 | unconjugated |
antibody form【抗体形式】 | purified immunoglobulin |
antibody product type | primary antibodies |
clone【克隆】 | HAP46.5, monoclonal |
form【形式】 | buffered aqueous solution |
mol wt【分子量】 | antigen ~25 kDa |
species reactivity | rat, mouse, chicken, canine, human |
technique(s) | indirect ELISA: suitable microarray: suitable western blot: 0.5-1 μg/mL using total cell extract of human MCF7 breast carcinoma cells |
isotype【同位素/亚型】 | IgG1 |
UniProt accession no.【UniProt登记号】 | P09429 |
shipped in【运输】 | dry ice |
storage temp.【储存温度】 | −20℃ |
Gene Information | human ... HMGB1(3146) mouse ... Hmgb1(15289) rat ... Hmgb1(25459) |
suitability【适用性】 | Monoclonal Anti-HMG-1 recognizes human, canine, mouse, rat, and chicken HMG-1. |
产品说明
Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
WGK | nwg |
Personal Protective Equipment【个人防护装备】 | Eyeshields, Gloves, multi-purpose combination respirator cartridge (US) |