Monoclonal Anti-H⁺/K⁺ ATPase (β Subunit) antibody produced in mouse

MFCD02096517

NA.41

The H⁺/K⁺-ATPase is a gastric proton pump that is a member of the P-type cation-transporting adenosine 5′-triphosphatase (ATPase) family. H⁺/K⁺-ATPase consists of a large transmembrane, catalytic α subunit, that contains sites for ATP binding and phosphorylation. This ATPase also has a smaller glycoprotein (β) subunit which may play a role in maintaining the structural and functional integrity of the complex. Mouse monoclonal anti-H⁺/K⁺ ATPase (β subunit) binds to H⁺/K⁺-ATPase β subunit in cow, dog, pig, rabbit, mouse, ferret and rat tissues. By immunoblotting, the antibody detects various forms of the β subunit, including a 60-80kDa glycosylated protein, a 52kDa β subunit precursor and the 34kDa core peptide.
The antibody reacts with various forms of the H⁺/K⁺ ATPase β subunit. It binds an epitope within amino acids 1-13 or 15-28 located on the cytoplasmic side of the β subunit. The antibody has been shown to inhibit the enzymatic activity of the H⁺/K⁺-ATPase and to alter the affinity of the cytoplasmic K⁺ binding site. It may be used to localize and detect H⁺/K⁺ ATPase β subunit.

core 34 kDa H⁺/K⁺ ATPase β subunit from deglycosylated pig gastric microsomes.

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
Mouse monoclonal anti-H⁺/K⁺ ATPase (β subunit) antibody can be used for indirect immunofluorescence (1:2,000), chemiluminescent western blot (1:4,000), and immunohistochemical applications.

Solution in phosphate buffered saline containing 0.05% sodium azide.

200

mouse

unconjugated

ascites fluid

primary antibodies

2G11, monoclonal

buffered aqueous solution

antigen 60-80 kDa (glycosylated form)

mouse, bovine, ferret, rabbit, canine, pig, rat

immunohistochemistry (frozen sections): suitable
indirect immunofluorescence: 1:2,000
western blot (chemiluminescent): 1:4,000

IgG1

P18597

dry ice

−20℃

mouse ... Atp4b(11945)
rat ... Atp4b(24217)

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