Monoclonal Anti-GOLPH3 antibody produced in mouse

200

mouse

purified immunoglobulin

primary antibodies

GOL3-1

buffered aqueous solution

antigen ~34 kDa

human, mouse, rat

~1 mg/mL

immunoblotting: 2.5-5 μg/mL using using whole extracts of rat PC-12 cells.
immunofluorescence: 2.5-5 μg/mL using mouse 3T3 cells
immunoprecipitation (IP): 5-10 μg using using lysates of human HeLa cells

IgG1

Q9H4A6

dry ice

−20℃

human ... GOLPH3(64083)
mouse ... Golph3(66629)
rat ... Golph3(78961)

GOLPH3 (golgi phosphoprotein 3) is an abundant phosphatidylinositol-4-phosphate (PtdIns₄P)-interacting protein which is conserved from yeast to humans. This protein is localized to Golgi through PtdIns₄P. This gene is localized to human chromosome 5p13. GOLPH3 has a molecular weight of 34kDa.
Monoclonal Anti-GOLPH3 (mouse IgG1 isotype) is derived from the hybridoma GOL3-1 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. GOLPH3 (Golgi phosphoprotein 3) is a peripheral membrane protein of the Golgi stack that localizes to the trans-Golgi network. GOLPH3 is conserved from yeast to human. This gene was described as an oncogene that is commonly amplified in human cancer and in cancer cell lines.

synthetic peptide corresponding to a sequence at the N-terminal region of human Golph3

Monoclonal Anti-GOLPH3 antibody produced in mouse has been used in:

• immunoblotting
• immunoprecipitation
• immunofluorescence

GOLPH3 (golgi phosphoprotein 3) links Golgi membrane to actin cytoskeleton by interacting with MYO18A, which is an unconventional myosin. This, in turn, is crucial for Golgi architecture and vesicle trafficking. It is an oncogene, which is susceptible to amplification in cancers, and is responsible for controlling the response to the anti-cancer drug, rapamycin. It is thought to function as a coat protein during Golgi trafficking.
GOLPH3 has a role in Golgi trafficking and morphology. It interacts with the unconventional myosin MYO18A, linking Golgi membranes to the actin cytoskeleton. GOLPH3 is also required for Golgi function. Overexpression of GOLPH3 is correlated with hyperactivation of mTOR signaling, in human cancer cells.

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

WGK 1