Monoclonal Anti-FUS antibody produced in mouse

NA.41

Monoclonal Anti-FUS (mouse IgM isotype) is derived from the hybridoma FUS-4 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to an internal sequence of human FUS, conjugated to KLH. Fused in sarcoma (FUS), also known as translocated in liposarcoma (TLS), ribonucleoprotein (RNP)-P2, amyotrophic lateral sclerosis-6 (ALS6), is a RNA/DNA binding protein. FUS is normally located predominantly in the nucleus whereas, pathological FUS inclusions are mostly found in the cytosol of neurons and glia cells.

synthetic peptide corresponding to an internal sequence of human FUS, conjugated to KLH. The corresponding sequence is identical in monkey and differs by 3 amino acids in mouse and rat.

Monoclonal Anti-FUS antibody produced in mouse has been used in various immunochemical techniques including:

• immunoblotting
• immunofluorescence
• immunohistochemistry

Fused in sarcoma (FUS) plays a vital role in transcription, RNA splicing and transport and is implicated in multiple diseases. Mutations in TAR DNA binding protein 43 (TDP-43) and FUS is associated with the development of amyotrophic lateral sclerosis (ALS) and fronto-temporal lobar degeneration (FLTD) including ubiquitin-positive inclusions (FLTDU). The majority of the FUS mutations has been recognized in C-terminal nuclear localization signal (NLS). FUS has been implicated in a broadening spectrum of neurodegenerative disorders. FUS has been identified as a component of inclusion bodies in patients with Huntington′s disease (HD) and spinocerebellar ataxias (SCA1-3).

Culture supernatant solution containing 15 mM sodium azide.

200

mouse

unconjugated

tissue culture supernatant

primary antibodies

FUS-4, monoclonal

buffered aqueous solution

antigen ~70 kDa

mouse, human, rat

immunohistochemistry: 1:500-1:1000 using formalin-fixed and paraffin embedded rat cerebellum.
indirect immunofluorescence: 1:200-1:400 using using HeLa or HepG2 cells.
western blot: 1:1000-1:2000 using lysates of G361 cells.

IgM

P35637

dry ice

−20℃

human ... FUS(2521)
mouse ... Fus(233908)
rat ... Fus(317385)

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12 - Non Combustible Liquids

WGK 2