MEF Ulk1 -/- Ulk2 -/- (DKO) (SIM)

mouse embryo

Mouse embryonic fibroblast, autophagy, Ulk1 -/- Ulk2 -/-, double knockout, spontaneously immortalised

Adherent

Fibroblast

cell culture | mammalian: suitable

dry ice

−196℃

Primary mouse embryonic fibroblasts (MEFs) were isolated from the embryos of a pregnant female Ulk1-/- Ulk2-/+ mouse at day 13.5 post coitus. The embryos were genotyped to identify those that were Ulk1 -/- Ulk2 -/- and the MEFs that were isolated and cultured were immortalised by a standard serial passaging protocol.

DMEM + 10% FCS + 2mM Glutamine

Trypsinize with 0.05% Trypsin/EDTA and inoculate new cultures at 2 x 10,000 cells per cm². Cultures must be incubated in a humidified 5% CO₂/95% air incubator at 37^oC

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Cultures from PHE Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.