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Magenta-Gal
产品编号: | 3106858 |
规格: | reagent for selection of recombinant bacterial clones |
CAS NO: | 93863-88-8 |
包装规格: | 25 MG |
产品类别: | 进口试剂 |
品牌: | Sigma-Aldrich |
优惠价: | 立即咨询 |
产品价格查看更多规格...
产品编号 | 包装单位 | 单价(元) | 国内现货 | 国外库存 | 询价单 |
3106858 | 25 MG | 3060 |
产品别名
93863-88-8
Magenta-Gal
5-Bromo-6-chloro-3-indolyl β-
Red-Gal
结构式
基本信息
Empirical Formula【经验(实验)分子式】 | C14H15BrClNO6 |
Molecular weight | 408.63 |
MDL number | MFCD02683897 |
PubChem Substance ID【PubChem化学物质编号】 | 329773145 |
NACRES | NA.85 |
General description【一般描述】 | Red-Gal is a chromogenic substrate for b-galactosidase, used to determine the presence or absence of a cloned DNA insert in bacteria growing on agar plates. Red-Gal is designed to replace X-Gal in the blue-white selection of recombinant bacterial colonies with the lac+ phenotype. Red-Gal is a chromogenic substrate for β-galactosidase, used to determine the presence or absence of a cloned DNA insert in bacteria growing on agar plates. Red-Gal is designed to replace X-Gal in blue-white selection of recombinant bacterial colonies with the lac+ phenotype. |
Application【应用】 | Magenta-Gal has been used for staining synthetic cytoplasmic or nuclear LacZ mRNA. It has also been used to detect the expression of the EphB1-β-gal fusion protein. ,Magenta-Gal is suitable for use in the selection of recombinant bacterial colonies with the lac+ phenotype. Chromogenic substrate for β-galactosidase producing a red or magenta insoluble product. |
Principle【原理】 | When Red-Gal is hydrolyzed by β-galactosidase, the resulting product will form a red precipitate. Lac+ colonies grown in the presence of Red-Gal turn an intense red color, allowing for easy differentiation between lac+ and lac- colonies. |
产品性质
Quality Level【质量水平】 | 200 |
grade【等级】 | for molecular biology |
sterility【无菌性】 | non-sterile |
product line【产品线】 | BioReagent |
Assay【测定】 | ≥98.0% (HPLC) |
form【形式】 | powder |
solubility【溶解性】 | methanol: soluble |
suitability【适用性】 | suitable for substrate for β-galactosidase |
storage temp.【储存温度】 | −20℃ |
SMILES string | OC[C@H]1O[C@H](Oc2c[nH]c3cc(Cl)c(Br)cc23)[C@H](O)[C@@H](O)[C@H]1O |
InChI | 1S/C14H15BrClNO6/c15-6-1-5-8(2-7(6)16)17-3-9(5)22-14-13(21)12(20)11(19)10(4-18)23-14/h1-3,10-14,17-21H,4H2/t10-,11+,12+,13-,14+/m1/s1 |
InChI key | CHRVKCMQIZYLNM-HTOAHKCRSA-N |
安全信息
Storage Class Code【储存分类代码】 | 11 - Combustible Solids |
WGK | WGK 3 |
Personal Protective Equipment【个人防护装备】 | Eyeshields, Gloves, type N95 (US) |