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基本信息
| eCl@ss | 32190102 |
| Cell Line Origin【细胞系来源】 | Human Caucasian lung squamous cell carcinoma |
| Cell Line Description【细胞系描述】 | Derived from a lung squamous cell carcinoma of a 72 year old Caucasian male. The B-lymphoblastoid cell line AGLCL (Sigma Catalogue number. 89120566) was derived from the same patient. Cells grow as large swollen aggregates, which will detach and eventually grow in suspension. |
| Application【应用】 | Control cell line for heterozygosity studies using the AGLCL cell line |
| Culture Medium【培养基】 | RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS). |
| Subculture Routine【传代培养常规】 | Split sub-confluent cultures (70-80%) 1:5 to 1:10 i.e. seeding at 5x1,000-2x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37℃. |
| Other Notes【其他说明】 | Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information. |
产品性质
| biological source【生物来源】 | human lung |
| growth mode【生长模式】 | Adherent |
| karyotype【核型】 | Not specified |
| morphology【形态学】 | Epithelial |
| products【产品】 | Not specified |
| receptors【受体】 | Not specified |
| technique(s) | cell culture | mammalian: suitable |
| relevant disease(s)【相关疾病】 | cancer |
| shipped in【运输】 | dry ice |
| storage temp.【储存温度】 | −196℃ |
