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产品性质
biological source【生物来源】 | human blood |
growth mode【生长模式】 | Suspension |
karyotype【核型】 | Not specified |
morphology【形态学】 | Lymphoblastoid |
products【产品】 | Not specified |
receptors【受体】 | Not specified |
technique(s) | cell culture | mammalian: suitable |
shipped in【运输】 | dry ice |
storage temp.【储存温度】 | −196℃ |
基本信息
Cell Line Origin【细胞系来源】 | Human lymphoblastoid, normal,EBV-transformed |
Cell Line Description【细胞系描述】 | Derived from mononuclear cells from peripheral blood of a 49 year old normal male. Cells were used as controls for cellular radiosensitivity studies and analysis of cell survival after UV-irradiation. The line LB173 has been established by subsequent transformation with EBV. Fibroblasts from the same individual have been designated 144BR. |
Application【应用】 | Mutation studies |
Culture Medium【培养基】 | RPMI 1640 + 4mM Glutamine + 200 μg/ml Sodium Pyruvate (NaPy) + 15% Heat Inactivated (HI-FBS). |
Subculture Routine【传代培养常规】 | Maintain cultures between 3-9 x 100,000 cells/ml, dilute every 1-2 days to 3 x 100,000 cells/ml; 5% CO2, 37℃. Cells form floating aggregates. |
Other Notes【其他说明】 | Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information. |