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HEPES solution, 1M
产品编号: | 3095875 |
规格: | Reagent designed and manufactured under cGMP controls suitable for use in an IVD application |
包装规格: | 100 ML,500 ML,1 L |
产品类别: | 进口试剂 |
品牌: | Sigma-Aldrich |
优惠价: | 立即咨询 |
产品别名
HEPES solution, 1M
HEPES Buffer
N-(2-Hydroxyethyl)piperazineN′-(2-ethanesulfonic acid)
产品性质
Quality Level【质量水平】 | 400 |
form【形式】 | liquid |
concentration【浓度】 | 1 M |
color【颜色】 | colorless |
pH【pH值(酸碱度)】 | 7.2-7.5 (25 ℃) |
application(s) | diagnostic assay manufacturing hematology histology |
storage temp.【储存温度】 | room temp |
基本信息
Application【应用】 | HEPES buffer preserves a normal physiological pH environment and is commonly used as a buffer in cell culture applications. HEPES buffer provides no nutritional benefit to cells, but adds extra buffering capacity to the cell culture particularly during experiments performed outside the carbon dioxide incubator. This 1 M stock solution contains HEPES adjusted to pH 7.35 with sodium hydroxide. Add the concentrated buffer to the cell culture medium to reach the desired concentration (typically 10–25 mM). Notes: If precipitation is present in the concentrated buffer, gently warm the bottle to 37 ℃ and mix until completely dissolved prior to dilution. |
安全信息
Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
WGK | WGK 1 |