您的位置：首页 > 产品中心 > GH3
产品性质
| biological source【生物来源】 | rat brain |
| growth mode【生长模式】 | Adherent |
| karyotype【核型】 | Hyperdiploid, modal no. 60 |
| morphology【形态学】 | Epithelial |
| products【产品】 | Growth hormone, prolactin |
| receptors【受体】 | Not specified |
| technique(s) | cell culture | mammalian: suitable |
| relevant disease(s)【相关疾病】 | cancer |
| shipped in【运输】 | dry ice |
| storage temp.【储存温度】 | −196℃ |
基本信息
| Cell Line Origin【细胞系来源】 | Rat Wistar-Furth pituitary tumour |
| Cell Line Description【细胞系描述】 | Derived from a 7 month female Wistar-Furth rat. GH3 cells produce growth hormone faster than the GH1 cell line and also produce prolactin. Hydrocortisone stimulates growth hormone production and inhibits prolactin production. Studies have shown that cells are capable of adaptation to suspension culture using MEM. |
| Application【应用】 | Hormone studies |
| Culture Medium【培养基】 | Ham′s F10 + 2mM Glutamine + 15% Horse Serum (HS) + 2.5% Foetal Bovine Serum (FBS). |
| Subculture Routine【传代培养常规】 | Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37℃. Upon thawing, the time taken before the first subculture can be performed may be extended (10-15 days). To aid attatchment, flask |
| Other Notes【其他说明】 | Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information. |
