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产品性质
| biological source【生物来源】 | human skin |
| growth mode【生长模式】 | Adherent |
| karyotype【核型】 | Triploid, modal n.o 69 |
| morphology【形态学】 | Epithelial |
| products【产品】 | Melanin |
| receptors【受体】 | Not specified |
| technique(s) | cell culture | mammalian: suitable |
| relevant disease(s)【相关疾病】 | cancer |
| shipped in【运输】 | dry ice |
| storage temp.【储存温度】 | −196℃ |
基本信息
| Cell Line Origin【细胞系来源】 | Human Caucasian malignant melanoma |
| Cell Line Description【细胞系描述】 | Established from a malignant melanoma of a 31 year old male Caucasian. The cells produce melanin for up to 50 population doublings. The Y chromosome could not be detected in this cell line by short tandem repeat (STR)-PCR analysis when tested at ECACC. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell line is identical to the source provided by the depositor based on the STR-PCR analysis. |
| Application【应用】 | Hormone studies |
| DNA Profile【DNA图谱分析】 | STR-PCR Data: Amelogenin: X CSF1PO: 10 D13S317: 11 D16S539: 15 D5S818: 11 D7S820: 8,11 THO1: 7,9 TPOX: 8,10 vWA: 15,17 |
| Culture Medium【培养基】 | McCoy′s 5a + 2mM Glutamine + 10% Foetal Bovine Serum (FBS). |
| Subculture Routine【传代培养常规】 | Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37℃. Cells are slow in attaching. |
| Other Notes【其他说明】 | Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information. |
