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产品性质
biological source【生物来源】 | human cervix |
growth mode【生长模式】 | Adherent |
karyotype【核型】 | Modal no. 71 |
morphology【形态学】 | Epithelial-like |
products【产品】 | Not specified |
receptors【受体】 | Not specified |
technique(s) | cell culture | mammalian: suitable |
relevant disease(s)【相关疾病】 | cancer |
shipped in【运输】 | dry ice |
storage temp.【储存温度】 | −196℃ |
基本信息
Cell Line Origin【细胞系来源】 | Human cervix carcinoma |
Cell Line Description【细胞系描述】 | Originally derived from normal amniotic tissue. Have been used and studied extensively including virus susceptibility, tumorigenicity and morphology. Contains HeLa marker chromosomes and exhibits type A G6PD. This cell line was found to be indistinguishable from HeLa by STR PCR DNA profiling. Therefore, the cell line should be considered as derived from HeLa. The cells are capable of growth in a variety of media supplemented with sera from several species. Ethnicity: Black. |
Application【应用】 | Virus, tumourigenicity and chromosome studies |
Culture Medium【培养基】 | EMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS). |
Subculture Routine【传代培养常规】 | Split sub-confluent cultures (70-80%) 1:3 to 1:5 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin; 5% CO2; 37℃. |
Other Notes【其他说明】 | Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information. |