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增强型禽逆转录酶 [eAMV TM RT]
Enhanced Avian Reverse Transcriptase [eAMVTM RT]
产品编号: | 3080999 |
规格: | For reverse transcription at higher temperatures & rare mRNAs |
包装规格: | 1000 UNITS |
产品类别: | 进口试剂 |
品牌: | Sigma-Aldrich |
优惠价: | 立即咨询 |
产品别名
增强型禽逆转录酶 [eAMV TM RT]
Enhanced Avian Reverse Transcriptase [eAMVTM RT]
基本信息
MDL number | MFCD01634820 |
NACRES | NA.55 |
Application【应用】 | Enhanced Avian Reverse Transcriptase可将RNA转录为DNA,同时显著提升mRNA模板合成cDNA的效率。这是由于本款增强型AMV-RT能够逆转录长链mRNA模板,能够有效穿越复杂的二级结构,可以通过RT-PCR反应检测低丰度mRNA。 |
Biochem/physiol Actions【生化/生理作用】 | 逆转录酶能够加速dNTP形成磷酸二酯键,从而催化RNA模板整合到互补DNA中。 |
Features and Benefits【特点和优势】 |
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Unit Definition【单位定义】 | 一单位酶能够以多聚腺苷酸为模板,以12-18的oligo(dT)为引物,在10分钟内将1纳摩TMP整合进TCA沉淀材料中。 |
Legal Information【法律信息】 | Purchase of this product is accompanied by a limited license for use in the Polymerase Chain Reaction (PCR) process for research purposes only and in conjunction with a thermal cycler whose use in the automated performance of the PCR process is covered by an up-front license fee, either by payment to Applied Biosystems or as purchased, i.e., and authorized thermal cycler. eAMV is a trademark of sigma-aldrich Co. LLC |
产品性质
Quality Level【质量水平】 | 200 |
form【形式】 | crystalline powder |
usage【用途】 | sufficient for 50 reactions |
feature【特点】 | dNTPs included: no hotstart: no |
concentration【浓度】 | 20 units/μL |
technique(s) | RT-PCR: suitable |
color【颜色】 | colorless |
input | purified RNA |
shipped in【运输】 | wet ice |
storage temp.【储存温度】 | −20℃ |
packaging【包装】 | 提供1管10x反应缓冲液。 |
安全信息
Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
WGK | WGK 3 |
Personal Protective Equipment【个人防护装备】 | Eyeshields, Gloves, multi-purpose combination respirator cartridge (US) |