DLD1 Cells PTEN -/-

NA.81

DLD1 Cells PTEN -/- are colorectal adenocarcinoma, epithelial cells, from a human adult male, with a ZFN knock out modification.
This product corresponds to ATCC Cat. No.CCL-221

Media Renewal changes two to three times per week
The cell freezing medium is DMSO 1X (Cat. No. C6164)
The base medium for this cell line is RPMI, Cat. No. R5886. To make the complete growth medium, add the following components to the base medium: fetal bovine serum, Cat. No. F4135, to a final concentration (v/v) of 10%.
Rapidly thaw vial by gentle agitation in 37℃ water bath (~2 minutes), keeping vial cap out of the water. Decontaminate with 70% ethanol, add 9 mL culture media and centrifuge 125 x g (5-7 minutes). Resuspend in complete culture media and incubate at 37℃ in a 5% CO2 atmosphere.
Subculture Ratio: approx. 1:3 to 1:10

These DLD-1 cells are adherent, with a doubling time of approx. 20 hours.
Zinc finger nuclease (ZFN) knock out on chromosome 10q23.31

Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification, PCR assay for cell line species confirmation.

组份可单独销售
DLD1 Control Cells 1 vial
DLD1 Cells PTEN -/- 1 vial

human male colon (Source Disease: Colorectal adenocarcinoma)

200

601728

−196℃

human ... PTEN(5728)