Cyanoborohydride Coupling Buffer

NA.56

Cyanoborohydride Coupling Buffer is used in affinity chromatography, protein chromatography, activated/functionalized matrices and synthetic reagents. Cyanoborohydride has been used to inform a safe and effective gene-transfer system targeting hepatocytes as well as to develop a method for targeted delivery of anticancer therapeutics to cancer cells in hypoxic areas.
A ready-to-use reagent used to couple amine ligands to aldehyde functional groups. The coupling buffer reaction is a reductive amination of the intermediate Schiff′s base to a stable C−N bond.
Cyanoborohydride Coupling Buffer has been used:

• in coupling reactions between amines and glutaraldehyde
• to reduce hydrazone bond to a stable hydrazide bond
• as a component in oligonucleotide reaction mixture for coverslips functionalization

Cyanoborohydride Coupling Buffer is a reagent suitable for reductive amination processes, that contributes to transformation of simple alcohols into more complex amines. It is used in the conversion of Schiff base, by reducing it, to form a secondary amine without affecting aldehyde groups on the support.

0.02 M sodium phosphate, pH 7.5, containing 0.2 M sodium chloride and 3.0 g/L sodium cyanoborohydride

reagent type: reductant

200

2-8℃

GHS07,GHS09

Warning

H302 - H411

P264 - P270 - P273 - P301 + P312 - P391 - P501

Acute Tox. 4 Oral - Aquatic Chronic 2

12 - Non Combustible Liquids

WGK 3