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Creatinine Deiminase microbial
产品编号: | 3072724 |
规格: | lyophilized powder, ≥25 units/mg protein |
CAS NO: | 37289-15-9 |
包装规格: | 250 UNITS |
产品类别: | 进口试剂 |
品牌: | Sigma-Aldrich |
优惠价: | 立即咨询 |
产品别名
37289-15-9
Creatinine Deiminase microbial
Creatinine Deaminase
基本信息
Enzyme Commission (EC) Number【酶学委员会(EC)编号】 | 3.5.4.21 ( BRENDA | IUBMB ) |
EC number【EC 号】 | 253-449-1 |
MDL number | MFCD00130878 |
NACRES | NA.54 |
Application【应用】 | Creatinine Deiminase microbial has been used:
Creatinine deiminase has been used in a study to assess the application of a creatinine-sensitive biosensor for hemodialysis control. Creatinine deiminase has also been used in a study to investigate the bioelectronic tongue for the simultaneous determination of urea, creatinine and alkaline ions in clinical samples. |
Biochem/physiol Actions【生化/生理作用】 | Creatinine deiminase catalyzes the hydrolysis of creatinine to methylhydantoine and ammonia. |
Physical properties【物理属性】 | Isoelectric point : 4.4 Michaelis constant : 3.5 x 10‾3M (Creatinine) Structure : 6 subunits per mol of enzyme Inhibitors : Ag+,Hg++, o-phenanthroline,monoiodoacetate Optimum pH : 8.5 – 9.5 Optimum temperature : 65 – 75℃ pH Stability : pH 7.0 – 11.0 (30℃, 20hr) Thermal stability : Below 65℃ (pH 7.5, 1hr) |
Unit Definition【单位定义】 | One unit will hydrolyze 1.0 μmole of creatinine to N-methylhydantoin and NH3 per min at pH 7.5 at 37 ℃ in a coupled system with |
Physical form【外形】 | Lyophilized powder containing mannitol as stabilizer |
产品性质
form【形式】 | lyophilized powder |
Quality Level【质量水平】 | 200 |
Specific Activity | ≥25 units/mg protein |
mol wt【分子量】 | ~260 kDa |
composition【组成】 | Protein, ≥15% biuret |
storage temp.【储存温度】 | −20℃ |
packaging【包装】 | 250 units in poly bottle |
安全信息
Storage Class Code【储存分类代码】 | 13 - Non Combustible Solids |
WGK | WGK 3 |
Personal Protective Equipment【个人防护装备】 | Eyeshields, Gloves, type N95 (US) |