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Creatinine Deiminase microbial

产品编号:3072724
规格:lyophilized powder, ≥25 units/mg protein
CAS NO:37289-15-9
包装规格:250 UNITS
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
3072724250 UNITS11740
产品别名

37289-15-9

Creatinine Deiminase microbial

Creatinine Deaminase

基本信息
Enzyme Commission (EC) Number【酶学委员会(EC)编号】
3.5.4.21 ( BRENDA | IUBMB )
EC number【EC 号】
253-449-1
MDL number
MFCD00130878
NACRES
NA.54
Application【应用】
Creatinine Deiminase microbial has been used:
  • to immobilize aminosilylated glass beads based biosensor for ammonia/ammonium and creatinine detection in urine
  • in creating creatinine-sensing membrane for biophysical studies
  • to investigate the bioelectronic tongue for the simultaneous determination of urea, creatinine and alkaline ions in clinical samples


Creatinine deiminase has been used in a study to assess the application of a creatinine-sensitive biosensor for hemodialysis control. Creatinine deiminase has also been used in a study to investigate the bioelectronic tongue for the simultaneous determination of urea, creatinine and alkaline ions in clinical samples.
Biochem/physiol Actions【生化/生理作用】
Creatinine deiminase catalyzes the hydrolysis of creatinine to methylhydantoine and ammonia.
Physical properties【物理属性】
Isoelectric point : 4.4
Michaelis constant : 3.5 x 10‾3M (Creatinine)
Structure : 6 subunits per mol of enzyme
Inhibitors : Ag+,Hg++, o-phenanthroline,monoiodoacetate
Optimum pH : 8.5 – 9.5
Optimum temperature : 65 – 75℃
pH Stability : pH 7.0 – 11.0 (30℃, 20hr)
Thermal stability : Below 65℃ (pH 7.5, 1hr)
Unit Definition【单位定义】
One unit will hydrolyze 1.0 μmole of creatinine to N-methylhydantoin and NH3 per min at pH 7.5 at 37 ℃ in a coupled system with L-glutamic dehydrogenase.
Physical form【外形】
Lyophilized powder containing mannitol as stabilizer
产品性质
form【形式】
lyophilized powder
Quality Level【质量水平】
200
Specific Activity
≥25 units/mg protein
mol wt【分子量】
~260 kDa
composition【组成】
Protein, ≥15% biuret
storage temp.【储存温度】
−20℃
packaging【包装】
250 units in poly bottle
安全信息
Storage Class Code【储存分类代码】
13 - Non Combustible Solids
WGK
WGK 3
Personal Protective Equipment【个人防护装备】
Eyeshields, Gloves, type N95 (US)
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